Description

AOD 9604 – Synthetic Peptide Fragment

PRODUCT SPECIFICATIONS

• Chemical Name: L-tyrosyl-L-leucyl-L-arginyl-L-isoleucyl-L-valyl-L-glutaminyl-L-cysteinyl-L-arginyl-L-seryl-L -valyl-L-alpha-glutamyl-glycyl-L-seryl-L-cysteinyl-glycyl-L-phenylalanine cyclic (7→14)-disulfide.
• 
• Peptide Classification: Synthetic growth-hormone–derived peptide fragment
• Peptide Type: Short-chain bioactive research peptide
• Molecular Weight: ~1814 Da (reported in literature)
• Purity: ≥99% (HPLC verified)
• Solubility: Soluble in sterile water and phosphate-buffered saline (PBS)
• Storage: −20 °C, protected from light and moisture
• Molecular Structure: AOD 9604 is a modified peptide fragment derived from growth hormone sequence research, engineered for enhanced structural stability and reproducible laboratory behavior.
• Disclaimer: For Research Use Only (RUO). Not for human or veterinary use.

MAIN DESCRIPTION

AOD 9604 is a synthetic growth-hormone–derived peptide fragment developed through rational peptide design to enhance molecular stability, enzymatic resistance, and laboratory reproducibility. Unlike full-length peptide hormones, AOD 9604 is engineered as a targeted research fragment to allow focused investigation of metabolic signaling mechanisms at the cellular level.

In biochemical research environments, AOD 9604 serves as a reference peptide model for pathway mapping, enzyme-regulated signal transduction, and intracellular metabolic simulation studies. Its predictable structural integrity and analytical consistency allow cross-laboratory replication and comparative peptide research.

EXPERIMENTAL & ANALYTICAL USE

AOD 9604 is used in laboratory research for:

• Mapping peptide–enzyme interaction pathways using in vitro assay platforms
• Studying intracellular metabolic signal modulation
• Evaluating structure–activity relationships (SAR) across synthetic peptide analogs
• Supporting biochemical modeling of enzyme-regulated pathways
• Comparative peptide framework analysis within metabolic signaling research

In these settings, AOD 9604 often serves as a reference peptide compound for benchmarking synthetic fragments across multi-peptide research systems.

Toxicology and Detection Methods

For toxicology and anti-doping research, AOD-9604 is detected in biological matrices using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Urinary Metabolite: The primary stable metabolite identified in urine is the sequence CRSVEGSCG, formed by the proteolytic cleavage of the N-terminal and C-terminal residues.

Detection Window: Methods utilizing solid-phase extraction (SPE) validated by Wiley have established a limit of detection (LOD) of 50 pg/mL, allowing for identification in urine samples for several days post-administration.

These analytical methods are used exclusively for forensic, anti-doping, and laboratory toxicology research and do not imply pharmacological application

Handling and Stability

AOD-9604 is typically supplied as a lyophilized white powder (acetate salt).

Solubility: It is slightly soluble in DMSO and water. Reconstitution often requires pH adjustment or the use of mild acetic acid to ensure full dissolution of the acetate salt form.

Storage: Lyophilized peptide is stable at -20°C. Once reconstituted, the presence of the disulfide bridge makes the solution sensitive to freeze-thaw cycles; aliquoting is recommended.

3D molecular structure of AOD-9604 (Tyr-hGH Frag 177-191) showing the cyclic disulfide bridge between Cys7 and Cys14. The ball-and-stick model represents the hexadecapeptide sequence C78H123N23O23S2 in a sterile laboratory setting, labeled for Research Use Only to distinguish from therapeutic products.

MOLECULAR & TECHNICAL CHARACTERISTICS

AOD-9604 is synthesized under high-purity (≥99%) HPLC-verified conditions, ensuring:

• Structural consistency
• Elimination of peptide chain truncation
• High aqueous solubility in standard buffers
• Stability during repeated analytical testing

When stored at −20 °C and protected from light and moisture, AOD-9604 retains its chemical integrity and laboratory performance stability, making it suitable for long-term research programs and cross-lab validation studies.

SCIENTIFIC SIGNIFICANCE

The study of AOD-9604 contributes to the broader understanding of:

• Peptide-regulated metabolic signaling
• Intracellular enzyme-driven modulation
• Synthetic peptide structure–function relationships
• Biochemical pathway modeling in preclinical research

By offering a chemically stable, reproducible, and ethically deployable peptide model, AOD 9604 enables researchers to investigate complex metabolic signaling behavior at the molecular level.

RUO COMPLIANCE

All experimental applications of AOD 9604 are conducted under Research Use Only (RUO) protocols.

It is not intended for human or veterinary use, nor for diagnostic or therapeutic purposes. All information is derived from peer-reviewed scientific literature and provided strictly for academic and laboratory reference.

FEATURES / HIGHLIGHTS

1. Synthetic growth-hormone–derived peptide fragment for metabolic signaling research
2. Supports intracellular enzyme-pathway investigation
3. Enables preclinical metabolic modeling under in vitro conditions
4. Verified ≥99% purity (HPLC-tested)
5. High solubility and long-term stability at −20 °C
6. Designed and certified for Research Use Only (RUO)

Why Profound Aminos

Profound Aminos supplies high-purity AOD 9604 (Tyr-hGH Frag 177-191) for controlled metabolic, adipocyte signaling, and cellular toxicology research programs.

Researchers value:

1. RUO-grade AOD 9604 with verified cyclic disulfide bridge integrity and purity validation.
2. Reliable solubility profiles and lyophilized stability for in vitro assays.
3. Mass and LC-MS identity verification to ensure experimental reproducibility.
4. Secure temperature-controlled packaging that maintains peptide conformational integrity.

Profound Aminos is selected by research organizations studying lipolytic pathways, beta-3 adrenergic receptors, energy homeostasis, and metabolic flexibility.

References

PubChem. Compound Summary: AOD 9604. National Library of Medicine.

FDA Pharmacy Compounding Advisory Committee. December 4, 2024 Meeting Briefing Document.

Cox, H.D., et al. Detection and in vitro metabolism of AOD9604. Drug Test Analysis.

World Anti-Doping Agency. The 2025 Prohibited List.

Heffernan, M., et al. The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism. PubMed.

STORAGE INSTRUCTIONS

1. Store at −20 °C in a sealed container
2. Protect from light and moisture
3. Avoid repeated freeze–thaw cycles
4. Maintained in a RUO-standard laboratory environment

FAQs

Q1: What is AOD 9604 used for?

AOD 9604 is used strictly in biochemical and molecular research to study peptide-driven metabolic signaling and intracellular enzyme interaction pathways.

Q2: Is AOD 9604 identical to growth hormone?

No. AOD 9604 is a synthetic peptide fragment derived from growth hormone sequence research, modified for laboratory stability and reproducible pathway investigation.

Q3: What purity is recommended for AOD 9604 research?

≥99% purity (HPLC verified) is recommended for consistent experimental outcomes.

Q4: Can AOD 9604 be used in humans or animals?

No. AOD 9604 is strictly for Research Use Only (RUO) and is not intended for diagnostic, therapeutic, or veterinary applications.

Q5: How should AOD 9604 be stored for long-term stability?

Store at −20 °C, away from light and moisture, to preserve structural integrity and RUO-grade purity.

RUO DISCLAIMER

This compound is for Research Use Only (RUO). It is not intended for human or veterinary applications. All information provided is based on peer-reviewed scientific literature and intended solely for laboratory and academic research purposes.